Isotopic Fractionation and Kinetic Isotope Effects of a Purified Bacterial Nitric Oxide Reductase (NOR).

ABSTRACT: Nitrous oxide (N₂O) is a serious concern due to its role in global warming and ozone destruction. Agricultural practices account for ∼80% of all anthropogenic N₂O produced in the US, due in large part to the stimulation of microbial denitrification. Stable isotopes are uniquely suited to examine both microbial N₂O sources and the mechanism of N₂O biosynthesis through the use of Site Preference (δ¹⁵N^SP; the difference in δ¹⁵N between the central and outer N atoms in N₂O) and kinetic isotope effects (KIEs), respectively. Using trace gas isotope ratio mass spectrometry (TG-IRMS), we determined the δ¹⁵N, δ¹⁵N^α, δ¹⁵N^β, and δ¹⁸O of N₂

SUBMITTER: Rivett ED 

PROVIDER: S-EPMC12548093 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature