Interaction of potato-derived RG-I with galectin-3 and gene regulation in human mesenchymal stem cells.

ABSTRACT:

Background

Mesenchymal stem cells (MSC) play a vital role in bone regeneration. Poly-(l-lactide-co-ε-caprolactone) scaffolds functionalised with modified rhamnogalacturonan-I (RG-I) potato pectin, can modulate inflammation and promote bone regeneration in-vitro and in-vivo by modulating the action of the galactoside-binding galectin-3. In this study, we determined the binding affinity to galectin-3 of potato unmodified RG-I (PU) and its arabinose-deficient form (PA) and investigated the transcriptomic effects of PA treatment on human MSCs evaluated through in-silico pathway analysis in relation to galectin-3.

Methods

The binding affinity of RG-I (PU and PA) to galectin-3 was assessed by tryptophan fluorescence spectroscopy. Human MSC (hMSC) isolated from the bone marrow of e

SUBMITTER: Mukhtar A 

PROVIDER: S-EPMC12548138 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature