Integrated analysis of single-cell RNA-seq and bulk RNA-seq reveal macrophage subpopulation characteristics and the role of STAT1 in rheumatoid arthritis.

ABSTRACT:

Background

Rheumatoid arthritis (RA) represents a persistent systemic autoimmune disorder characterized by chronic inflammation and significant disability. Macrophage heterogeneity plays a crucial role in the progression of RA, but the molecular characteristics and regulatory mechanisms of its subsets have not yet been fully elucidated.

Methods

In this study, we integrated single-cell RNA sequencing (scRNA-seq) data and bulk RNA sequencing (bulk RNA-seq) data, and employed multiple strategies for analysis and identification of the heterogeneity of macrophages in RA. We identified STAT1 as a key gene through a combined approach utilizing LASSO regression and random forest models. The differential expression of STAT1 was subsequently validated in an adjuvant-induced arthritis

SUBMITTER: Ye L 

PROVIDER: S-EPMC12548165 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature