Development of a Protocol for Long-Term Ex Vivo Normothermic Machine Perfusion of Rodent Livers.

ABSTRACT:

Introduction

Long-term normothermic machine perfusion (LT-NMP) enables the assessment and optimization of livers for days and, potentially, weeks. However, models of LT-NMP have only been described for human and pig livers, which are resource intensive and impractical for laboratory research. Cost-effective small animal models of LT-NMP are needed for future research. This study aimed to develop a system for LT-NMP of rat livers for up to 72 h.

Methods

This study was performed in two stages: the development phase (n = 20) and validation phase (n = 5). The perfusion system included an organ reservoir, pump, heat exchanger, long-term oxygenator, and dialysis. Hormonal and nutritional support were continuously infused. During the validation phase, five consecutive grafts were p

SUBMITTER: Ly M 

PROVIDER: S-EPMC12548313 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature