Simultaneous Ex Situ Normothermic Perfusion of Paired Kidneys in Pigs.

ABSTRACT:

Background

Normothermic Machine Perfusion (NMP) is essential in renal transplantation to improve organ viability before transplantation. This study presents a proof of concept for simultaneous ex situ perfusion of paired porcine kidneys using exogenous creatinine and iohexol clearances to assess renal function, with the primary objective of examining intra-individual differences.

Methods

Five kidney pairs (n = 10) were harvested from pigs, preserved at 4°C for 3 h, and subjected to 6-h NMP. Each pair was perfused with a solution containing red blood cells, and perfusion parameters were monitored continuously. Biochemical parameters were assessed using hourly perfusate and urine samples. Kidney function was evaluated using creatinine, which was introduced during the priming p

SUBMITTER: Navez M 

PROVIDER: S-EPMC12548330 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature