Modular Virus Capsid Coatings for Biocatalytic DNA Origami Nanoreactors.

ABSTRACT: Protein cages and custom DNA structures have emerged as self-assembling nanocompartments to sequester enzymes and mimic the compartmentalization of naturally occurring biocatalytic reactions. Protein cages excel in gating the interaction between enzyme and substrate, which can be affected by the physicochemical properties of protein units, whereas the high addressability of DNA origami allows stoichiometric control over the enzyme loading and precise positioning of enzymes. Nevertheless, both approaches would benefit from overcoming the challenges related to controlled enzyme loading and substrate flux, which could be resolved by combining the two nanomaterials. Here, we assemble virus capsid proteins on an enzyme-loaded DNA origami nanoreactor in a modular manner and demonstrate size-sele

SUBMITTER: Seitz I 

PROVIDER: S-EPMC12548342 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature