Dual regulatory role of hsa-miR-122b-5p in chikungunya virus infection via interaction with CHIKV 3'-UTR and HDAC4 modulation.

ABSTRACT: Establishment of a viral infection in the host involves complex interactions between the virus and the host. The present study was undertaken to evaluate one such interaction between a host microRNA (miRNA) with both the virus and its cellular target. Through a comprehensive high-throughput screen of a human miRNA mimics library, we identified that hsa-miR-122b-5p bound to chikungunya virus (CHIKV) 3'-untranslated region (3'-UTR), which we abrogated upon mutating the binding sites of the miRNA and determined that this miRNA regulated CHIKV infection. In-depth scrutiny of this miRNA's expression in CHIKV-susceptible host cells divulged that it was mainly expressed in macrophages, and temporal expression profiling analysis in these cells revealed that its expression negatively correlated to

SUBMITTER: Srivastava P 

PROVIDER: S-EPMC12548407 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature