Residues 27T and 297A in VP2 contribute to the enhanced replication and pathogenicity of raccoon dog parvovirus.

ABSTRACT: Raccoon dog parvovirus (RDPV) is a highly contagious pathogen causing severe hemorrhagic enteritis that is fatal in young raccoon dogs. Since 2016, epidemiological investigations have documented recurrent outbreaks of RDPV, exhibiting heightened virulence; however, the molecular mechanisms driving this increased pathogenicity remain poorly understood. In this study, an alignment of 67 complete RDPV sequences identified two high-frequency amino acid mutations at positions 27 and 297 in the VP2 capsid protein that distinguish RDPV strains from before and after the 2016 outbreak. We generated a series of variant viruses with single or double amino acid substitutions using infectious cDNA clones from RDPV/CL/2016 (rRDPV-16) and RDPV/FLD/2010 (rRDPV-10) strains. In F81 cells, the mutant virus d

SUBMITTER: Xu L 

PROVIDER: S-EPMC12548415 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature