Effects of low intracellular glutathione and CbrA-CbrB-Crc signaling on methylglyoxal sensitivity in <i>Pseudomonas aeruginosa</i> LasR-deficient mutants.

ABSTRACT: Pseudomonas aeruginosa, which causes many types of infections, often has loss-of-function mutations in the gene encoding LasR, a transcription factor involved in quorum sensing. Here, we report that LasR- strains are more sensitive to the electrophile methylglyoxal (MGO), which is produced by many cells, including those involved in immune defense. MGO, due to its high reactivity, must be rapidly detoxified to prevent the formation of damaging adducts on macromolecules. GloA3 is a GSH-dependent MGO glyoxalase involved in MGO detoxification, and we found that intracellular GSH levels were lower in ∆lasR strains, as measured by multiple methods. Furthermore, we show that exogenous GSH and GloA3 overexpression both enhance GloA3-dependent MGO resistance, particularly in a ∆las

SUBMITTER: Altamirano A 

PROVIDER: S-EPMC12548427 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature