The E3 ubiquitin ligase STUB1 inhibits Senecavirus A replication by mediating VP1 ubiquitination and proteasomal degradation.

ABSTRACT: Senecavirus A (SVA), an emerging vesicular pathogen, poses a significant threat to the global pig industry. STIP1 homology and U-box-containing protein 1 (STUB1), a chaperone-dependent E3 ubiquitin ligase, plays a pivotal role in protein quality control by mediating target protein degradation. However, its precise role of STUB1 in regulating SVA replication remains undefined. In this study, we combined liquid chromatography-mass spectrometry, confocal imaging, and Western blotting to demonstrate that STUB1 interacts with the SVA VP1 protein and negatively regulates SVA replication. Mechanistically, STUB1 promotes the ubiquitination-dependent degradation of VP1 by specifically targeting lysine residues at positions 177 and 260 (K177 and K260). This degradation process is significantly enhan

SUBMITTER: Zeng P 

PROVIDER: S-EPMC12548440 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature