Hepatitis C virus modified _SE2_F442NYT-mRNA-LNP candidate vaccine promotes helper CXCR5⁺T cells.

ABSTRACT: T cells play an important role in initiating antibody responses by instructive signals of cell-cell contacts and secretion of soluble cytokines as mediators. We investigated the role of the modified soluble E2 (sE2_F442NYT) antigen from hepatitis C virus (HCV) on healthy human peripheral blood mononuclear cell (PBMC)-derived immune cells or immunized mouse cells to understand the mechanisms of immune regulation by the candidate vaccine antigen. HCV E2 and E2_F442NYT displayed a role in inducing type 17 T-helper cell (Th17) phenotype, as indicated by interleukin-17 (IL-17) expression and signal transducer and activator of transcription 3 (Stat3) phosphorylation. The spleen cells from sE2-mRNA-lipid nanoparticles (LNPs) or sE2_F442NYT-mRNA-LNP-immunized mice exh

SUBMITTER: Haga Y 

PROVIDER: S-EPMC12548457 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature