Segregation of sister chromosomes during the shape change of developing <i>Myxococcus xanthus</i> cells.

ABSTRACT: Chromosome organization is critical for the maintenance of genetic integrity. Most studies of bacterial nucleoids have focused on growing rod-shaped organisms. Studying nucleoid dynamics during Myxococcus xanthus development offers the unique opportunity to investigate the localization of two sister chromosomes as rod-shaped cells transition into round spores. During starvation-induced multicellular development, DNA replication is required for M. xanthus rods to transition into spores with two copies of the chromosome. Here, we report novel approaches using confocal fluorescence microscopy to observe the chromosome number and arrangement, and nucleoid localization in developing cells in situ. We discovered that sister chromosomes are present in some rods and transition

SUBMITTER: Hoang Y 

PROVIDER: S-EPMC12548468 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature