Replication-competent adenovirus reporters utilizing endogenous viral expression architecture.

ABSTRACT: Adenoviruses are double-stranded DNA viruses widely used as platforms for vaccines, oncolytics, and gene delivery. However, tools for studying adenoviral gene expression in real time during infection remain limited. Here, we describe a set of fluorescent and bioluminescent reporter viruses built using the modular AdenoBuilder reverse genetics system and informed by high-resolution maps of Ad5 transcription. These reporters utilize endogenous early and late transcriptional units, enabling visualization of viral gene expression without exogenous promoters or splicing elements. These model viruses replicate with kinetics nearly indistinguishable from wild-type virus and have enabled real-time fluorescent imaging as well as longitudinal bioluminescent sampling from the same infected samples. T

SUBMITTER: O'Brien CM 

PROVIDER: S-EPMC12548470 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature