Deep Learning for Hyperpolarized NMR of Intrinsically Disordered Proteins without Resolution Loss: Access to Short-Lived Intermediates.

ABSTRACT: The inherently low sensitivity of solution-state Nuclear Magnetic Resonance (NMR) has long limited its ability to characterize transient biomolecular states at atomic resolution. While dissolution dynamic nuclear polarization (dDNP) offers a compensating signal enhancement, its broader use has been hindered by rapid polarization decay, causing severe spectral distortion. Here, we introduce HyperW-Decon, an approach that enables high-sensitivity, high-resolution NMR of biomolecules in solution. HyperW-Decon combines two key aspects: (i) the use of hyperpolarized water (HyperW) to transfer polarization to proteins through rapid proton exchange; and (ii) a theory-driven, machine learning (ML)-based deconvolution method that corrects polarization-induced artifacts without requiring external re

SUBMITTER: Turhan E 

PROVIDER: S-EPMC12548504 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature