Exploring the Relationship Between Biofilm Formation and Antibiotic Resistance Genes in Clinically Isolated <i>Klebsiella pneumoniae</i>.

ABSTRACT: Persistent pathogens pose a significant global health burden, contributing to increased morbidity and mortality rates worldwide. This study investigates the relationship between clinically relevant biofilm-associated and antibiotic resistance genes in Klebsiella pneumoniae isolates. Biofilm-forming capabilities of the isolates were evaluated, and their biomass was quantitatively analyzed. The presence of biofilm-associated and resistance genes (mrkA, blaSHV, blaTEM, and blaKPC) in the samples was identified using conventional PCR. Gene expression levels were quantified via RT-qPCR under acidic and neutral pH conditions, and the results were analyzed statistically to evaluate significance. All clinical isolates were found to be biofilm formers. PCR analysi

SUBMITTER: Abdulqadir HN 

PROVIDER: S-EPMC12549196 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature