Differential impact of manganese on glutamate clearance, electroencephalography, and sleep in Alzheimer's disease.

ABSTRACT:

Introduction

Underlying glutamate dysregulation in Alzheimer's disease can be worsened by environmental factors like manganese (Mn) exposure. This study examined how excess Mn affects glutamatergic signaling and neurotransmission in a beta-amyloid mouse model.

Methods

APP/PSEN1 and control mice were exposed to systemic Mn subcutaneously. Gene expression, glutamate clearance dynamics, electroencephalography (EEG) activity, and sleep architecture were analyzed using quantitative polymerase chain reaction (qPCR), Western blot, ex vivo hippocampal slices, and EEG recordings.

Results

Mn exposure elevated brain Mn levels and altered glutamate dynamics in both WT and APP/PSEN1 mice. Wild-type (WT) mice showed faster glutamate clearance, increased spiking, disrupted sleep, an

SUBMITTER: Buchanan RA 

PROVIDER: S-EPMC12549221 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature