Application of next-generation sequencing for detecting <i>Mycoplasma</i> contamination in veterinary vaccines.

ABSTRACT: Ensuring the safety and efficacy of veterinary vaccines requires reliable methods for detecting microbial contamination, particularly from Mycoplasma species, which pose a significant risk in cell-culture-derived vaccines. In the Republic of Korea, polymerase chain reaction (PCR) is predominantly used for Mycoplasma testing due to its faster turnaround compared to culture-based methods. However, in combination with vaccines containing Erysipelothrix rhusiopathiae and classical swine fever virus, PCR is rendered ineffective because of cross-reactivity between Mycoplasma universal primers and E. rhusiopathiae, resulting in non-specific amplification. This limitation necessitates reliance on the labor-intensive culture method, underscoring the need for more

SUBMITTER: Go SM 

PROVIDER: S-EPMC12549248 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature