Construction of a novel cuproptosis-related gene signature for predicting microenvironment, prognosis and therapeutic response in cervical cancer.

ABSTRACT:

Introduction

Cervical cancer is a common malignant tumor in females, and its carcinogenesis needs further elucidation. Cuproptosis is a novel mode of cell death and its role in cervical cancer is largely unknown.

Methods

The data of 334 cases of cervical cancer patients were extracted from public databases, including TCGA, GEO, GSCA and Msigdb databases. The R package, Kaplan-Meier and Cox regression analysis were used to construct the prediction model. To confirm the validation of the model, FOXJ1 was over-expressed in HeLa and SiHa cells. CCK-8, EdU, colony formation and Transwell assays were used to test the proliferation, invasion and migration abilities. Western blotting was utilized to examine the changes of protein levels.

Results

We constructed a six-gene sign

SUBMITTER: Cui Y 

PROVIDER: S-EPMC12549250 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature