Immunomodulatory inhibition of osteoclastogenesis by a marine microalgal ethanol fraction targeting T-cells, antigen presentation, and macrophage fate.

ABSTRACT:

Background

Targeting immune pathways to prevent bone loss represents a promising, yet underexplored therapeutic strategy.

Methods

An ethanol-soluble fraction derived from the freeze-dried biomass of the marine microalga Skeletonema costatum (SKLT) was tested for its ability to modulate immune responses and inhibit osteoclastogenesis. Its effects were evaluated in a zebrafish model of bone regeneration, a medaka model of RANKL-induced osteoporosis, and in vitro using murine RAW 264.7 macrophages. Transcriptomic profiling of regenerating fin blastemas at 24 hours post-amputation was performed to identify the affected molecular pathways.

Results

In zebrafish, SKLT treatment suppressed the recruitment of osteoclast precursors and altered mineralization dynam

SUBMITTER: Carletti A 

PROVIDER: S-EPMC12549262 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature