<i>In vivo</i> functional screening on innate immunity of lactic acid bacteria in <i>Galleria mellonella</i> preclinical model: comparative analysis of <i>Lactiplantibacillus plantarum</i> and <i>Lentilactobacillus kefiri</i>.

ABSTRACT:

Introduction

Among probiotics, Lactic Acid Bacteria modulate host immunity via strain-specific molecular patterns. The invertebrate model Galleria mellonella offers conserved innate immune pathways and is increasingly applied for preclinical screening of probiotic functions.

Methods

We evaluated the immunomodulatory activity of Lactiplantibacillus plantarum ATCC 14917 and Lentilactobacillus kefiri DSM 10551 in G. mellonella. Larvae were injected with 10⁶ CFU/larva of each strain, and survival and health indices were monitored for 72 h. The temporal transcriptional response of ten innate immunity-related genes, including Toll and IMD signalling, Toll receptor, cytokine-like ligand, and antimicrobial effectors, was assessed via qRT-PCR ov

SUBMITTER: Guarnieri A 

PROVIDER: S-EPMC12549655 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature