Label-free photoacoustic imaging of glassfrog development.

ABSTRACT: A primary focus of contemporary biology is to understand how internal molecules influence natural development. Many amphibians serve as highly effective model organisms for this research due to their rapid growth rates and transparent tissues, which facilitate high-resolution imaging. In our research, we utilized two complementary photoacoustic microscopy (PAM) configurations: hyperspectral PAM (HS-PAM) and ultrafast functional PAM (UFF-PAM). HS-PAM enabled us to achieve cellular-level resolution in vitro, while UFF-PAM allowed us to capture hemodynamic changes of adult specimens in vivo. We monitored the morphological changes in glassfrogs from neurulation to the tadpole stage by detecting a variety of intrinsic contrasts, including DNA/RNA, yolk proteins, lipids, hemoglobin

SUBMITTER: Rong Q 

PROVIDER: S-EPMC12549789 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature