The WW domain presents a promising target for the development of PCIF1 agonists in the treatment of glioma.

ABSTRACT: PCIF1 is currently the sole m6Am methyltransferase in mammalian mRNA, but it was initially named for the interaction between its WW domain and RNA polymerase II. Emerging studies reveal the important role of PCIF1 in the progression of various cancers, particularly highlighting its methyltransferase activity. Recently, we found that PCIF1 effectively inhibited the growth of gliomas both in vitro and in vivo. Notably, this inhibition was not entirely dependent on its methyltransferase activity. In this study, we found that the deletion or mutation of the WW domain reversed the inhibitory effect of PCIF1 on cell proliferation in both PCIF1 wild-type and knockout glioma cells. Overexpression of the WW domain of PCIF1 alone significantly inhibited glioma cell proliferation without notable effe

SUBMITTER: Yin J 

PROVIDER: S-EPMC12549845 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature