A constraint-based framework for exploring the impact of multireaction dependencies on metabolic functions.

ABSTRACT: Metabolism operates under physico-chemical constraints that result in multireaction dependencies. Understanding how multireaction dependencies affect metabolic phenotypes remains challenging, hindering their biotechnological applications. Here, we propose the concept of a forcedly balanced complex that allows to efficiently determine the effects of specific multireaction dependencies on metabolic network functions in constrained-based models. Using this concept, we found that the fraction of multireaction dependencies induced by forcedly balanced complexes in genome-scale metabolic networks followed power law with exponential cut-off. We identified forcedly balanced complexes that are lethal in cancer but have little effect on growth in healthy tissue models. In addition, these forcedly ba

SUBMITTER: Kuken A 

PROVIDER: S-EPMC12549881 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature