Characterization of core promoter activation by the Drosophila insulator-binding protein BEAF.

ABSTRACT: Housekeeping and regulated genes have distinct core promoter motif usage and architecture. The Boundary Element-Associated Factor of 32 kDa, BEAF, was identified as a chromatin domain insulator protein that affects chromatin structure and plays a role in insulator function. Genome-wide mapping found that it usually binds near transcription start sites of housekeeping genes found at topologically associating domain (TAD) boundaries, suggesting roles in both insulator function and gene activation. This was substantiated when it was found to activate the RpS12 and aurA promoters, and that BEAF-dependent promoter activation could be separated from BEAF-dependent insulator activity. Here, we use luciferase assays after transient transfection of Drosophila S2 cells to show that adding an upstrea

SUBMITTER: Negedu S 

PROVIDER: S-EPMC12549938 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature