DNA computing function switching by programming base stacking interactions with minimal molecular architecture changes.

ABSTRACT: In biological systems, molecular network functionalities are usually switched in a flexible, facile, and programmable manner. Mimicking this, substantial studies are directed towards developing synthetic DNA networks that exhibit similar function-switching capabilities, though often hindered by extensive molecular architecture changes and stringent condition controls, which result in a time-consuming and labor-intensive process. Here, we develop a base stacking-mediated allostery strategy to manipulate the DNA computing function switching with minimal molecular architecture changes, usually as few as 1-2 nucleotide changes. We implement up to 20 distinct logic function switching within DNAzyme networks. We also validate our function switching platform to implement totally 84 kinds of gene

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12549992 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature