MRNA recruitment by G3BP1 condensates is regulated by Caprin1 but requires G3BP1 binding to mRNA.

ABSTRACT: Upon stress exposure, cells repress translation and release mRNAs from polysomes. The sudden increase in cytoplasmic messenger RNA (mRNA) concentration triggers the formation of cytoplasmic condensates known as stress granules (SGs) that enrich mRNA and RNA binding proteins (RBPs). SGs assemble through liquid-liquid phase separation involving interactions between a subset of RBPs and mRNA. G3BP1 is considered the central participant of this network and functions as a molecular switch depending on the mRNA concentration and the recruitment of G3BP1 protein partners. Among them, Caprin1 is an SG associated protein that binds the NTF2-L domain of G3BP1, promoting SG assembly. Herein, we show that Caprin1 triggers the formation of large G3BP1-mRNA condensates in vitro and improves both the mRN

SUBMITTER: Lavrynenko K 

PROVIDER: S-EPMC12550004 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature