A comparison of commercial assays quantifying mature brain-derived neurotrophic factor (mBDNF) and its precursor (pro-BDNF) in human serum.

ABSTRACT: Brain-derived neurotrophic factor (BDNF) and its isoforms (pro- and mBDNF) are promising neurobiomarkers. While total serum BDNF levels have been previously validated, the newer isoforms have not. We aimed to identify the most common serum pro- and mBDNF assays through a literature search (MEDLINE/PubMed, until March 2024), and to compare their methodological performance in 23 human serum samples [total BDNF: R&D Systems, #DBNT00; both isoforms: R&DSystems (#DY3175, #DBD00); Aviscera-Bioscience (#SK00752-09, #SK00752-01); FineTest (#EH4255, #EH0043)]. Western-blot and cross-reactivity assays were used to confirm whether the kits tested for the declared isoforms. The total BDNF (#DBNT00) and pro-BDNF (#DY3175) ELISA kits from R&D Systems, and the pro-BDNF ELISA kits from FineTest (#EH4255)

SUBMITTER: Olivas-Martinez A 

PROVIDER: S-EPMC12550038 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature