A cortex-wide multimodal microscope for simultaneous Ca²⁺ and hemodynamic imaging in awake mice.

ABSTRACT: Developments in optical imaging techniques have advanced the study of neurovascular coupling across the whole cortex. Unfortunately, each cortex-wide optical imaging modality is specialized in revealing limited neural or hemodynamic actions. Here, we develop a cortex-wide multimodal microscope (multiScope) integrating widefield Ca²⁺ fluorescence microscopy, optical resolution photoacoustic microscopy, and laser speckle contrast imaging to simultaneously observe neuron firing, total hemoglobin and blood flow velocity with endogenous biomarkers. The multiScope features a cortex-wide field-of-view of Ø 8.6 mm, a maximum imaging speed of 4 Hz, and the average spatial resolutions of 10.7 ± 3.1 μm and 7.1 ± 0.8 μm respectively for widefield imaging and photoacoustic microscopy after m

SUBMITTER: Qin W 

PROVIDER: S-EPMC12550049 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature