Zoonotic potential of methicillin-resistant Staphylococcus aureus isolated from pets and their owners in Bangladesh.

ABSTRACT: This study investigated the prevalence of methicillin-resistant Staphylococcus aureus (MRSA), their antimicrobial resistance, virulence determinants, and potential zoonotic potential between pets, owners, and veterinary professionals. From July 2022 to June 2024, 213 human and 191 animal samples (nasal, pus, and wound swabs) were collected from two veterinary hospitals. Staphylococcus aureus was isolated using conventional cultural method and subsequently confirmed through Gram staining, biochemical tests, and PCR targeting the nuc gene. Methicillin resistance was confirmed using the cefoxitin disk diffusion method and PCR for the mecA gene. Antimicrobial susceptibility testing was performed using the disc diffusion method against 15 antibiotics. Additionally, whole genome sequencing (WGS)

SUBMITTER: Sarker H 

PROVIDER: S-EPMC12550073 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature