Primed human pluripotent stem cell-derived blastocyst-like cell aggregates with partial lineage specification.

ABSTRACT:

Introduction

Human blastoids, blastocyst-like structures assembled in vitro, hold great promise for decoding the earliest steps of human development. However, most existing protocols rely on naïve human pluripotent stem cells (hPSCs), which are chromosomally unstable and technically demanding.

Methods

We developed a method to generate human blastocyst-like cell aggregates directly from primed hPSCs using a thermoresponsive hydrogel. Generated blastocyst-like cell aggregates were evaluated for morphological features, lineage marker expression via immunocytochemistry, transcriptional profiles using single-cell RNA sequencing, and functional capacity through in vitro implantation assays.

Results

Primed hPSC-derived blastocyst-like cell aggregates recapitula

SUBMITTER: Mutsuda-Zapater K 

PROVIDER: S-EPMC12550238 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature