Mapping the structural dynamics of red- and blue-emitting beetle luciferases revealed by HDX-MS.

ABSTRACT: Beetle luciferases catalyze bioluminescent reactions using identical substrates-luciferin, ATP, and oxygen-yet emit colors ranging from green to red. As the luciferase fold is highly conserved, the structural basis of this spectral diversity remains elusive. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) was used to systematically probe the conformational dynamics of green-emitting Amydetes vivianii luciferase (GB_Av) and red-emitting Phrixothrix hirtus luciferase (RE_Ph), along with their color-shifting mutants, R337L and L334R, respectively. HDX-MS profiling in the apo and ligand-bound states mapped dynamic changes associated with substrate binding and color modulation. RE_Ph and the red-shifted R337L GB_Av mutant exhibited gl

SUBMITTER: Kharbatli AR 

PROVIDER: S-EPMC12550276 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature