Enrichment of Helminth Mitochondrial Genomes From Faecal Samples Using Hybridisation Capture.

ABSTRACT: New approaches are urgently needed to enrich rare or low-abundant DNA in complex samples. Soil-transmitted helminths (STHs) inhabit heterogeneous environments, including the gastrointestinal tract of their host as adults and are excreted as eggs and larvae in faeces, complicating our understanding of their biology and the use of genetic tools for species monitoring and population tracking. We have developed a hybridisation capture approach to enrich mitochondrial genome sequences of two STH species, the roundworm Ascaris lumbricoides and whipworm Trichuris trichiura, from extracted DNA from faecal material and worm specimens. Employing ~1000 targeted probes, we achieved > 6000 and > 12,000 fold enrichment for A. lumbricoides and T. trichiura, respectively, relative to direct whole genome s

SUBMITTER: Papaiakovou M 

PROVIDER: S-EPMC12550466 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature