The Nuclear Receptor Nurr1 Modulates the Expression and Activity of PPARγ in Human Pro-Inflammatory Macrophages.

ABSTRACT: Nurr1 is a member of the nuclear receptor family NR4A that modulates inflammation typically by inhibiting the NF-κB signalling pathway. In vitro, Nurr1 can interact with the peroxisome proliferator-activated receptor (PPAR)γ, as well as be recruited to the Pparg promoter in microglial cells; however, their functional relationship is not established. Here, we aimed to investigate the role of Nurr1 on PPARγ activity in human macrophages. Blood monocytes were cultured with GM-CSF or M-CSF to generate pro- (GM-MDMs) and anti-inflammatory (M-MDMs) macrophages, respectively. The protein levels of PPARγ and Nurr1 were elevated in GM-MDMs compared to M-MDMs, and their expression was positively correlated. PPARγ activation in GM-MDMs with the agonist rosiglitazone did not modify Nurr1 expression. H

SUBMITTER: Santana-Cisneros E 

PROVIDER: S-EPMC12550473 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature