Probing the Dynamic Strength of Biomolecular Interactions with Single-Cell Centrifugation.

ABSTRACT: Molecular interactions between receptors and ligands govern critical biological processes, from immune surveillance and T-cell activation to tissue development. However, current techniques for studying binding avidity often sacrifice throughput or precision. We introduce a high-throughput method for quantifying molecular and cellular binding kinetics using a centrifuge force microscope (CFM)a compact imaging system integrated into a benchtop centrifuge. The CFM performs real-time force measurements on thousands of single cells in parallel, probing receptor-ligand interactions under controlled mechanical stress. To extend these capabilities, we developed a next-generation CFM with dual-channel fluorescence imaging that enables tracking of individual cell unbinding events. To demonstrate it

SUBMITTER: Bergal HT 

PROVIDER: S-EPMC12550632 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature