Generation Using Phage-Display of pH-Dependent Antibodies Against the Tumor-Associated Antigen AXL.

ABSTRACT:

Background/objectives

Tumor-associated antigens are not tumor-specific antigens but proteins that are overexpressed by tumor cells and also weakly expressed at the surface of healthy tissues. Therefore, some side effects are observed when targeted by therapeutic antibodies, a phenomenon named "on-target, off-tumor toxicity". As tumors generate an acidic microenvironment, we investigated whether we could generate pH-dependent antibodies to increase their tumor specificity. For this proof-of-concept study, we selected the tyrosine kinase receptor AXL because we already developed several antibodies against this target.

Methods

To generate a pH-dependent anti-AXL antibody, we performed classical panning of a single-chain variable fragment (scFv) library using phage display at an

SUBMITTER: Mangeat T 

PROVIDER: S-EPMC12550914 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature