Temporal and Spatial Profiling of <i>Escherichia coli</i> O157:H7 Surface Proteome: Insights into Intestinal Colonisation Dynamics In Vivo.

ABSTRACT:

Background

EHEC O157:H7 causes severe gastrointestinal illness by first colonizing the large intestine. It intimately attaches to the epithelial lining, orchestrating distinctive "attaching and effacing" lesions that disrupt the host's cellular landscape. While much is known about the well-established virulence factors, there are much to learn about the surface proteins' roles in a living host.

Methods

This study presents the first in vivo characterisation of the surface proteome, i.e., proteosurfaceome, of Escherichia coli O157:H7 EDL933 during intestinal infection, revealing spatial and temporal adaptations critical for colonisation and survival. Using a murine ileal loop model, surface proteomic profiles were analysed at early (3 h) and late (10 h) infection stages

SUBMITTER: Monteiro R 

PROVIDER: S-EPMC12551062 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature