Concentrated Growth Factor Induces ER Stress and Apoptosis by Increasing Ceramide Generation in Selected Tumour Cell Lines.

ABSTRACT: Concentrated growth factor (CGF), a blood-derived autologous biomaterial, is increasingly utilised in regenerative medicine and, recently, in cancer-related surgeries. Rich in cytokines, platelets, nucleated cells and fibrin scaffolds, CGF offers therapeutic promise but requires rigorous safety evaluation in oncology. This study explores the effects of CGF-conditioned medium (CGF-CM) on breast cancer (MCF7, MDA-231) and osteosarcoma (SaOS-2, MG-63) cell lines. Our findings reveal that CGF-CM selectively induces cytotoxic effects in MCF7 and SaOS-2 cells, while no cytotoxicity was observed in MDA-231 and MG-63 cells. Early apoptosis in MCF7 and SaOS-2 cells was accompanied by mitochondrial dysfunction, evidenced by an increased BAX/BCL-2 ratio and cytochrome c release. CGF-CM treatment also

SUBMITTER: Palermo A 

PROVIDER: S-EPMC12551144 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature