Decoding the human tongue of light chain amyloidosis in three dimensions (3D): three-dimensional pathological visualization drives early and precise screening of suspected cases.

ABSTRACT:

Objectives

This study aimed to apply CUBIC tissue clearing combined with three-dimensional (3D) imaging to visualize the pathological architecture of systemic light chain (AL) amyloidosis in the human tongue, assess spatial and microstructural changes associated with amyloid deposition, and explore its potential to enhance early diagnostic accuracy.

Materials and methods

Seven tongue specimens from confirmed AL amyloidosis cases underwent CUBIC processing, multiplex immunofluorescence staining, and 3D imaging. Vascular density, surface area, vessel and muscle fiber diameters were compared between amyloid deposition and non-deposition regions. Ten additional specimens initially negative for AL amyloidosis were re-evaluated using the same procedure, suspected cases were verifi

SUBMITTER: Wu L 

PROVIDER: S-EPMC12551279 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature