SPP1^high macrophage-induced T-cell stress promotes colon cancer liver metastasis through SPP1/CD44/PI3K/AKT signaling.

ABSTRACT:

Background

Patients with colon cancer liver metastases (CCLM) frequently exhibit poor responses to immunotherapy, a phenomenon attributed in part to an immune desert tumor microenvironment. This study aimed to comprehensively characterize the immune landscape in primary colon cancers and their matched liver metastases via single-cell transcriptome analysis, with the goal of identifying potential immunotherapeutic targets.

Methods

Tumor specimens from patients with CCLM were subjected to single-cell RNA sequencing. Immune subpopulations were profiled with emphasis on exhausted T cells (Tex)-including both CD8⁺ and CD4⁺ ANK3⁺ subsets-as well as on a distinct stress response T-cell subset (TSTR) defined by high HSPA1A/HSPA1B expression. In paral

SUBMITTER: Ding D 

PROVIDER: S-EPMC12551530 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature