Murine vascular organoids are responsive and adaptable 3D systems with cellular heterogeneity and dynamic plasticity.

ABSTRACT: Blood vascular organoids (BVOs) have emerged as powerful tools for interrogating endothelial heterogeneity, function, and organ-specific cues during development, homeostasis, and disease. Here, we describe the stepwise generation and characterization of murine BVOs from different embryonic stem cells and introduce a fluorescent tracer system to follow their formation and maturation, visualizing functional and perfused vessels after transplantations into mice. Single-cell RNA sequencing, FACS, and whole-mount immunohistochemistry revealed a heterogeneous and dynamic trajectory of multiple endothelial, mesenchymal, and macrophage clusters in mBVOs at growth spike and full maturation. Endothelial cells inferred high plasticity toward arterial, venous, capillary, and tip/stalk cells and a neur

SUBMITTER: Guelfi S 

PROVIDER: S-EPMC12551706 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature