A 3D spheroid model for assessing nanocarrier-based drug delivery to solid tumors.

ABSTRACT: 3D spheroid culture has emerged as a valuable tool for studying complex intratumoral processes and screening novel therapeutics in vitro. However, spheroids face reproducibility and data interpretation issues, which limit their utility. This work describes a simple and reproducible co-culture spheroid model compatible with high-throughput screening designed to study pancreatic ductal adenocarcinoma (PDAC), a highly therapy-resistant cancer. These spheroids, composed of both cancer and stromal cells, recapitulate key features of PDAC which are difficult to study in traditional 2D cell culture, including hypoxia, fibrosis and chemoresistance. Light sheet microscopy is used to study the tissue penetration of polymeric Pluronic® F127-polydopamine (PluPDA) nanocarriers (NCs) in this model while

SUBMITTER: Yadav C 

PROVIDER: S-EPMC12552133 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature