Glycan synthesis with SpyCatcher-SpyTag immobilized Leloir-glycosyltransferases.

ABSTRACT: Human milk oligosaccharides (HMOs) have been proven to play key roles in human development and health benefits. Further research requires high-purity HMOs in large quantities. While enzymatic glycan synthesis has been proven to be highly selective and efficient, it is often associated with costly enzyme production and loss of enzymes during synthesis. Immobilization of enzymes can counteract this disadvantage, making synthesis more cost-effective. In this study, we report a versatile immobilization technique for Leloir-glycosyltransferases (GTs) onto maleimide-activated agarose by employing the SpyCatcher-SpyTag technology. The five GTs: human β1,4-galactosyltransferase 1 (β4GalT), Neisseria meningitidis β1,3-N-acetylglucosaminyltransferase (LgtA), Escherichia coli O55:H7 β1,3-galactosyltr

SUBMITTER: Palm P 

PROVIDER: S-EPMC12552311 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature