Use of cesium chloride density gradient ultracentrifugation for the purification and characterization of recombinant adeno-associated virus.

ABSTRACT: Recombinant adeno-associated virus (rAAV) has been widely used as an effective delivery tool in gene therapy. One of the challenges facing the production of high-quality rAAV is optimization of the production and purification methodologies. Cesium chloride density gradient ultracentrifugation (CsCl-DGUC) has been traditionally utilized for rAAV purification; however, few studies have focused on CsCl-DGUC for rAAV purification despite this technique having a great potential for clinical-grade or large-scale rAAV production. In this study, we aimed to explore the unaddressed challenges associated with rAAV purification using CsCl-DGUC. We clarified that AAV capsids assembled by the different stoichiometries of three viral proteins (VP1, VP2, and VP3) showed heterogeneous population in the Cs

SUBMITTER: Hirohata K 

PROVIDER: S-EPMC12552358 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature