Effects of natural deep eutectic solvents' hydration level, choice of hydrogen bond donor and application of ultrasound on the extraction, anti-nutritional components, structural properties and functionality of canola protein isolates.

ABSTRACT: In a systematic attempt to improve the cost-effectiveness and knowledge of the topic of plant protein extraction using natural deep eutectic solvents (NDESs), 18 extraction treatments, half of which paired with sonication, were performed to extract canola protein isolates using two groups of NDESs: choline chloride-glucose-water (CG) and choline chloride-water (C), with water contents ranging from 30 to 90 %. The highest protein extraction efficiencies were observed at water contents of 60 % for CG group (50.28 %) and 90 % for C group (45.48 %), while sonication improved these efficiencies to 60.68 % and 58.11 %, respectively. Increased solvent hydration and sonication also effectively reduced phenolic compounds and phytic acid contents, especially in the CG group. Despite increasing the s

SUBMITTER: Karimi A 

PROVIDER: S-EPMC12552561 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature