Alkaline phosphatase (ALPL) as a diagnostic and prognostic biomarker linked to immune response in thyroid cancer.

ABSTRACT:

Background

Thyroid cancer (THCA) is a rapidly increasing endocrine malignancy. This study aims to explore alkaline phosphatase (ALPL) as a potential prognostic biomarker and therapeutic target, focusing on its role in tumor progression and immune infiltration.

Methods

We utilized RNA sequencing (RNA-Seq) data from The Cancer Genome Atlas (TCGA) to investigate the role of ALPL in THCA. Comprehensive analyses included differential gene expression, survival outcomes, immune cell infiltration, and RNA methylation correlations. Functional assays such as cell counting kit-8, colony formation, Transwell migration/invasion, 5-ethynyl-2'-deoxyuridine (EdU) staining, and flow cytometry were conducted to evaluate the effects of ALPL knockdown in THCA cell lines. At

SUBMITTER: Gao M 

PROVIDER: S-EPMC12552581 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature