Meningeal lymphatic architecture and drainage dynamics surrounding the human middle meningeal artery.

ABSTRACT: The anatomical basis of cerebrospinal and interstitial fluid clearance along the human ventral meninges remains poorly defined. Here, we present the in vivo and ex vivo evidence of a distinct CSF-ISF drainage compartment surrounding the middle meningeal artery (MMA) in humans. In five healthy participants, dynamic contrast-enhanced MRI revealed delayed signal enhancement along the MMA-peripheral region, peaking at 90 min, substantially later than adjacent vascular and lymphatic structures, consistent with slower, nonvascular clearance dynamics. To validate these findings, we performed the high-resolution spatial mapping of the dorsal and ventral dura using immunofluorescence and imaging mass cytometry, identifying PROX1+, PDPN+, and LYVE1+ lymphatic-like structures that are a

SUBMITTER: Albayram M 

PROVIDER: S-EPMC12552907 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature