A Redundant System of Thioredoxin and Glutathione Is Essential for Pancreatic Acinar Integrity.

ABSTRACT:

Background & aims

Oxidative stress and antioxidant defense mechanisms have long been implicated in the pathogenesis of acute pancreatitis (AP). However, there is a notable lack of in vivo experimental evidence clarifying their precise role.

Methods

We generated and analyzed mice with a pancreas-specific deletion of Txnrd1 (Txnrd1^Δpanc). AP was induced in these mice using cerulein injections. Pancreatic tissue was subsequently analyzed using immunoblotting, histology, immunohistochemistry, RNA sequencing, and biochemical assays.

Results

Txnrd1^Δpanc mice exhibited normal growth, pancreatic weight, histology, and pancreatic function comparable to controls, although they experienced a slightly more severe course of AP. An increase in glutathione leve

SUBMITTER: Einwachter H 

PROVIDER: S-EPMC12553062 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature