Temperature-independent and photocrosslinkable hydrolyzed GelMA with tunable mechanics for biofabrication.

ABSTRACT: Thermoresponsive and photo-crosslinkable hydrogels are highly desirable for biofabrication; however, conventional gelatin methacryloyl (GelMA) exhibited thermal gelation below 30 °C and high viscosity, restricting its compatibility with concentration-dependent tunability and lower critical solution temperature-type systems. To address these issues, hydrolyzed GelMA (hGelMA) was developed by subjecting GelMA to controlled enzymatic degradation, effectively eliminating thermal gelation while maintaining photo-crosslinkability and enabling broader control of the mechanical properties through concentration adjustment. The resulting hGelMA was characterized for viscosity, mechanical tunability, and compatibility with digital light processing bioprinting. Cell compatibility was evaluated, and th

SUBMITTER: Kyeong J 

PROVIDER: S-EPMC12553068 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature