Ortho2Web: a workflow for disentangling the roles of hybridization and allopolyploidization in reticulation within Campanulaceae.

ABSTRACT:

Background

Phylogenetic networks (Web of Life) depict the intricate evolutionary dynamics of organisms more accurately than simple bifurcating trees (Tree of Life), because processes such as hybridization and polyploidy connect lineages through reticulation. However, most existing workflows still examine evolutionary mechanisms separately. To provide an integrated view, we studied the bellflower tribe (Campanuleae), a lineage with a well‑documented reticulate past, and developed Ortho2Web, a workflow that teases apart hybridization and polyploidization within a single analytical framework.

Results

Ortho2Web employs multiple strategies to reduce the impact of non-biological factors, including (1) integrating multi-source genomic data to minimize sampling gaps, (2) generating

SUBMITTER: Xu C 

PROVIDER: S-EPMC12553268 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature