Intra-host evolution of cell-fusing agent virus following acute infection in <i>Aedes aegypti</i> mosquito.

ABSTRACT: While intra-host evolution of arboviruses in mosquitoes has been documented, studies of insect-specific viruses (ISVs) remain limited. This study examines evolutionary patterns [i.e. evolutionary process, mutational types (synonymous/nonsynonymous)] of the cell-fusing agent virus (CFAV), an ISV that infects adult Aedes aegypti, over a period of 21 days post-infection (dpi), with a focus on the relationship between viral population dynamics and genetic diversity. High-throughput sequencing of amplification products covering the entire viral genome revealed a significant positive correlation of CFAV genetic diversity with viral population size and natural selection ([Formula: see text]/[Formula: see text]). Notably, diversity for both synonymous and nonsynonymous single nucleotide var

SUBMITTER: Uddin MM 

PROVIDER: S-EPMC12553305 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature